Nucleotide sequence analysis of the Leptospira biflexa serovar patoc rpsL and rpsG genes.
نویسندگان
چکیده
The Leptospira biflexa rpsL and rpsG genes were sequenced. Although similar in many respects, proteins encoded by these L. biflexa genes had several unusual features when compared with homologous proteins of other organisms. Unlike the rpsL genes of other eubacteria, the L. biflexa rpsL gene is adjacent to a rpoC-like gene.
منابع مشابه
Identification Leptospira santarosai serovar shermani specific sequences by suppression subtractive hybridization.
In Taiwan, leptospirosis is caused mainly by Leptospira santarosai serovar shermani. Suppression subtractive hybridization was employed to isolate DNA fragments present in pathogenic L. santarosai serovar shermani but absent in non-pathogenic L. biflexa serovar patoc. Analysis of 23 subtracted DNA clones revealed 25 gene fragments by BLASTX program. Eight clones showed similarity to transposase...
متن کاملCytopathic effect in BHK 21 (C13) cells inoculated with Leptospira interrogans serovar Pomona isolated from a porcine abortion.
BHK 21 (C13) cells inoculated with a wild strain of Leptospira interrogans serovar Pomona isolated from a porcine abortion exhibited cythopathic effect (CPE) consistent in degenerative morphological alterations whereas no cell changes or alterations were observed in cells infected with a non-pathogenic Leptospira biflexa serovar Patoc strain. BHK 21 (C13) cells were seeded on 24 well cell cultu...
متن کاملIdentification of clinical isolates of Leptospira spp by pulsed field gel-electrophoresis and microscopic agglutination test.
Twenty-five clinical isolates of Leptospira spp were characterized by microscopic agglutination test (MAT) and pulsed field gel-electrophoresis (PFGE) in comparison with 23 reference Leptospira serovars and with the saprophytic L. biflexa serovar Patoc. PFGE DNA profiling was more specific and reliable than MAT.
متن کاملrpoB gene analysis as a novel strategy for identification of spirochetes from the genera Borrelia, Treponema, and Leptospira.
Spirochetes are emerging pathogens for which culture and identification are partly unresolved. In fact, 16S rRNA-based sequencing is by far the most widely used PCR methodology that is able to detect such uncultivable pathogens. However, this assay actually has some limitations linked to potential problems of contamination, which hampers diagnosis. To circumvent this, we have devised a simple P...
متن کاملAntileptospiral activity of xanthones from Garcinia mangostana and synergy of gamma-mangostin with penicillin G
BACKGROUND Leptospirosis, one of the most widespread zoonotic infectious diseases worldwide, is caused by spirochetes bacteria of the genus Leptospira. The present study examined inhibitory activity of purified xanthones and crude extracts from Garcinia mangostana against both non-pathogenic and pathogenic leptospira. Synergy between γ-mangostin and penicillin G against leptospires was also det...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Journal of bacteriology
دوره 172 10 شماره
صفحات -
تاریخ انتشار 1990